Studies on the Precipitin Reaction

Quantitative Studies on the Precipitin Reaction Effect of Salts on Tee Reaction*

In the studies on the precipitin reaction hitherto published from this laboratory (1) the salt concentration was held constant at 0.9 per cent of sodium chloride in order to permit observations on the effect of varying the proportions of antigen (or hapten) and antibody. In the present paper are reported data obtained during several years on the influence on the course of the precipitin reactio...

Quantitative Studies on the Precipitin Reaction Effect of Salts on the P~eaction*

In the studies on the precipitin reaction hitherto published from this laboratory (1) the salt concentration was held constant at 0.9 per cent of sodium chloride in order to permit observations on the effect of varying the proportions of antigen (or hapten) and antibody. In the present paper are reported data obtained during several years on the influence on the course of the precipitin reactio...

Nephelometric studies of the precipitin reaction: a model system for specific protein measurements.

Light-scattering studies have been carried out on an immunochemical system that serves as a model for specific protein determinations in biological samples. The influence of antigen/antibody ratio, salts, urea, pH, temperature, and excitation wavelength are presented. Of significance is the finding that ions, particularly F-, increase the rate of formation of and the light scattering from the a...

Equine Antiserum Precipitin Reaction with VII. The Quantitative on Tobacco Mosaic Virus: Immunochemical Studies

Characterization of the "reactant" (gamma Globulin Factor) in the F Ii Precipitin Reaction and the F Ii Tanned Sheep Cell Agglutination Test

The material in gamma globulin ("reactant") which reacts with rheumatoid sera in the F II precipitin and F II tanned sheep cell agglutination tests was concentrated by precipitation with sodium sulfate. The concentrated "reactant" appeared to consist of polydispersed molecular aggregates of gamma globulin with s(20) constants as high as 40, as well as the previously described inhomogeneous aggr...

Antinuclear antibody with distinct specificity for polymyositis.

In the course of studying antinuclear antibodies in the rheumatic diseases, a new precipitin reaction (provisionally referred to as PM-1) was observed between calf thymus nuclear extract and polymyositis sera. Objectives of this study were to further define the immunologic nature of this reaction and to determine its specificity for polymyositis. Immunodiffusion studies using calf thymus nuclea...